Coding

Part:BBa_K3982002

Designed by: Akankshya Ramkrishna Sahu   Group: iGEM21_IISER_Berhampur   (2021-10-01)


Tobacco Etch Virus (TEV) protease

Tobacco Etch Virus (TEV) protease is used in Project CODE M by Team IISER_Berhampur 2021


Usage and Biology

TEV protease is a highly specific protease from Tobacco Etch Virus. It is used for controlled cleavage of fusion proteins and thus, their easy purification in in-vitro and in-vivo experiments. The cleavage site is between Gln and Gly/Ser residues.


Reference

Harrington, L. B., Burstein, D., Chen, J. S., Paez-Espino, D., Ma, E., Witte, I. P., Cofsky, J. C., Kyrpides, N. C., Banfield, J. F., & Doudna, J. A. (2018). Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. Science (New York, N.Y.), 362(6416), 839–842. https://doi.org/10.1126/science.aav4294


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None